The flexibility of UV-inducible mutation in Deinococcus ficus as evidenced by the existence of the imuB–dnaE2 gene cassette and generation of superior feather degrading bacteria Introduction This era of the genome has brought in an unprecedented opportunity to engineer microorganisms for a multitude of diverse biotechnological applications. To accomplish this, the work and research requires rigorous scientific approaches rooted in methodical experimental design and execution. This paper explores the SOS pathway of TLS polymerase, mutagenesis and the generation of superior keratin breakdown pathways. Where polymerase I-III can stall at UV damage such as thymine dimmers in DNA strands, polymerase IV/V or TLS, Translesion polymerase, is able to move along and repair the damage, however by doing this it also induces mutation, a method of survival to continue the replication cycle of DNA. The team then continues to explore these genes by performing knockouts to deduce that the i...