S-STEM Scholar Micheal Dockham's Blog

 Monday 11.18           14 flask, 2 for each pH level were inoculated with WT d. ficus and left in the shaking incubator at      37C for 24 hours. Tuesday 11.19 Inoculated flasks were checked upon arrival of lab, most media showed minimal to zero growth so far. Left to incubate for another 24 hours.  Wednesday 11.20     Flask were removed from incubator after 48hr of growth.  OD Values of flask were then quantified. Growth was seen at pH 7, 7.23(c) and 8. Growth was not detected at pH 5,6,9 and 10. OD values were highest at the pH of 8 with an OD value of 3.21.  Control media, pH 7.23 had an OD of 2.94 and the grwoth in pH 7 measured an OD of 2.58      Thursday 11.21 Day was spent helping Madi calculate and plan for her pH experiment. We decided to use 3 pH levels to start, 6, 7 and 8, with the possibility of going father in both directions in the future. We discussed the avenues of this research a...

 Monday 11-11           Tuesday 11-12              After compiling a list of materials and receiving them, I got to work making my pH controlled media. At first, the proper amount of each acid and base was added, then dissolved in 80mL of di H2O This was done for every pH level, using the appropriate amount of materials, then dialed in using HCl and NaOH, then filled to 100mL total. Each flask had a concentration of 12mM. These were then covered and put together with the rest of the autoclave items. Wednesday 11-13 Received all 7 flask (pH level each) from autoclave and divided them out into 3x 30mL flask each, with 10mls left for testing. Autoclave showed to have minimal effect on most buffer solutions, while pH 9 and 10, sodium carbonate/bicarbonate, showed significant color change. They also showed the most change in pH between pre and post-autoclave testing,  Thursday 11-14 Sick Friday 11-15 Sick