D. Ficus Transformation Preperations.
This week has spent planning and prepping for the transformation of D. Ficus. 4.29 TGY media for plates was too concentrated agar wise, remade to 500ml from 250ml and re-autoclaved. 4.30 Poured 14 TGY plates for future inoculation. 2 TGY plates were inoculated w. D. Ficus cells were from February, may not grow. Need to observe. Made 5x flask of 50ml of LB broth for future E. Coli inoculations. .1ml/flask of amp(25mg/ml) 5.1 Inoculated 2x 20ml TGY flask with D. Ficus after positive gram stain results Inoculated 2x 50ml LB flask with E.Coli, added 100ul of 25mg/ml amp. Discussed further plans of plasmid extraction and component cell protocols and material request. Planned for transformation next week. 5.2 Gram stained all cultures, both E. coli and D. Ficus All good results Took OD values of chosen cultures. E. Coli 2 OD 600 = 2.10 D. Ficus 2 OD 600 = 2.93 Held Biotech Club Meeting Prepped for Plasmid Extractions and Component Cell Protoco...