Week of 5.28.24 - Cont. D. ficus Transformation

 Tuesday 5.28

  • Prepped TGY broth for cultures (5x 20ml)
  • Prepped TGY agar for plates (300ml)
  • Discussed experiment and transformation with Dr. Tuohy
    • Experimental Plates, (Predicted Result)
      • TGY, (+)
      • TGY +cmr, (+)
    • Control Plates
      • TGY, (+)
      • TGY +cmr, (-)
Wednesday 5.29
  • Poured TGY plates - 6
  • Poured tgy +cmr 3ug/mL - 2
  • Performed Transformation protocol - pRAD1 one uptake into D. ficus

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