D. Ficus Transformation Cont. & Contamination Concerns

 5.7.24

        Spent majority of the lab prepping and running gel to confirm the presence of pRAD1 plasmid in plasmid extraction performed the week before hand.

Electrophoresis Gel - 30ml @1% with new dye

    2ul - Plasmid

    8uL - PCR H20

    2uL - Dye

    6uL - 1Kb Ladder

Gel ran @ ~70mA for just under an hour. 

This left us with an unusual band amount of 4. Where typically we see 3 bands, a supercoiled plasmid, linear plasmid and circular plasmid, here we see four. Went home to research and worked with team to and decided to check plasmid extraction kit for contamination.  It appears I have a multimer, and have been suggested to linearize my plasmid via restriction site and then run a gel again. Will approach team with this idea next week. 




5.8 
Plasmid extraction kit contamination results. 


No contamination found in reagents. 



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