Week of 8.19 - 8.23

 Began performing "carnage test" on aliquoted D. ficus culutres stored in the -80C

Trest was to find optimal settings with the machine, based on our theory that the more "carnage" the higher the likelihood of transformation success. 

    40ul from -80C were thawed and placed in the cuvette for electroporation.

        Settings were:

            3000V

            25uF

           Infinite resistance, which uses then natural resistance of the cells versus a dialed in resistance.

            Cells were then diluted and plated, placed in the 37C for 24 hours and observed the next day

            No "carnage" was observed and plate had full growth.


Later in week performed experiment again on 2 samples with different settings, to observe any differences after shock. 

            #1 2400V

                    100Ohms

                    50uF

            #2 3000V

                    100Ohms

                    50uF

Two samples were shocked at different settings to observe and differences in growth after shock.

Later we found that the "shock-pod' was broken and not functioning properly. This was concluded while working with the BioRad rep who was helping us diagnose the problems. 

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